Generating primary murine vaginal fibroblast cell lines
نویسندگان
چکیده
منابع مشابه
Fibroblast Cell Lines
Murine 3T6 cells selected in increasing concentrations of methotrexate were unstable with respect to dihydrofolate reductase overproduction and methotrexate resistance when they are cultured in the absence of methotrexate. An analysis of the karyotypes of these resistant cells revealed the presence of numerous double minute chromosomes. We observed essentially identical kinetics of loss of dihy...
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ژورنال
عنوان ژورنال: MethodsX
سال: 2020
ISSN: 2215-0161
DOI: 10.1016/j.mex.2020.101100